Temperature Control System for Biological Tissues in Electroporation Studies

It is known that biological tissues present temperature-dependent electrical conductivity. This fact has been considered in electroporation-based cancer therapies such as electrochemotherapy and ablation with irreversible electroporation, especially in pre-treatment simulations, mathematical modeling, and the development of new protocols. In this work, we proposed a novel low-cost experimental setup suitable for plate electrodes based on Peltier modules to control the temperature of the tissue sample while electroporation protocols are assessed in the laboratory. The setup was implemented and tested with liver tissue samples at two temperature setpoints. Measurements results showed the expected behavior in the electrical properties of the electroporated samples, demonstrating the feasibility of the proposed setup for conducting electroporation measurements while stabilizing its temperature.